KIT MIDI FLOW ILLUSTRA BLOOD GENOMICPREP 1 * 25 Tes (GE HEALTHCARE)

ویرایش
90.5.27

تاریخ اعتبار
90.7.27

لطفا پس از پایان اعتبار قیمت با شماره تلفن 88748085 تماس حاصل فرمائید

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Technical Information


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Lanes 1, 3, 5 represent the samples digested with EcoRI for 2 h.
Lanes 2, 4, 6 represent genomic DNA used in the digestion experiments.

The illustra™ blood genomicPrep Midi Flow Kit can be used to purify genomic DNA from human whole blood, buffy coat, and animal blood such as horse, sheep, rat, mouse, guinea pig, and chicken whole blood. The kit provides yields of up to 250 μg of high-quality genomic DNA. The kit is robust, highly reproducible, and versatile allowing you to choose either gravity or spin methods with input sample volumes of 1 to 8 ml. The genomic DNA isolated with the illustra blood genomicPrep Midi Flow Kit is suitable for most biological applications including PCR, real-time PCR, restriction enzyme digest, and sequencing. The kit uses a simple and efficient three-step process involving a two-step lysis procedure at ambient temperature, anion-exchange column chromatography, and a highly reproducible desalting process. The kit contains fast-flow genomic 250 columns, lyophilized proteinase K powder, lysis solutions, load and elution buffers, NAP-25 desalting column, spin column adapters, a full protocol booklet, and a detachable, quick reference protocol card.

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Pulse-field gel electrophoresis showing high-quality genomic DNA isolated from human whole blood using the illustra blood genomicPrep Midi Flow Kit and a QIAGEN blood & cell culture midi kit. Lanes 1 and 2 are lambda and low-range markers, Lanes 3 to 14 represent genomic DNA purified with the illustra blood genomicPrep Midi Flow Kit, and Lanes 15 to 19 represent genomic DNA purified with a Qiagen blood & cell culture midi kit.

TECHNICAL SPECIFICATIONS
blood Midi (1 to 8 ml; 5 ml optimum) 
Yield 85 to 120 μg 
Purity 1.7 to 1.9 
Total time of prep* 2 h 
Hands-on time* 45 min 
Size > 50 kb 
*including lysis, purification & desalting
فروشنده: آزمیران
گارانتی اصالت و سلامت کالا
پشتیبانی ۷ روز هفته ۲۴ ساعته
۸۲۰,۰۰۰ تومان
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