تایوگلیکولات فلوید مدیا ساخت شرکت میکرومدیا مجارستان 500 گرمی

Description of MM0069


 

                             Thioglycollate Medium USP

 

 

Use and description:  

For the cultivation of anaerobic, microaerophilic and aerobic microorganisms in sterility testing formulation recommended by USP. The medium can be used for confirmation test sin the enumeration of Clostridium perfringens in food products.  The reducing agents (thioglycollate, cystine) decrease the redox potentional and cystine helps the growth of fastidious anaerobes. It also neutrolizes arsenic, mercury and other heavy metal compounds. Resazurine is used as oxidation-reduction indicator; the medium is colourless int he reduced state and becomes pink when oxidised.

 

Composition per liter:

Peptones…………………………………….15.000 g

Glucose………………………………………5.500 g

Yeast extract…………………………………5.000 g

Sodium chloride……………………………...2.500 g

Agar………………………………………….0.750 g

L-cystine……………………………………..0.500 g

Sodium thioglycollate………………………..0.500 g

Resazurine……………………………………0.001 g

 

Final pH of the ready to use medium:   7.1 ± 0.2

 

Medium preparation:  

Add 30.0 grams of dehydrated culture medium to 1 liter of distilled water. Mix thoroughly. Gently heat and bring to the boiling. Distribute into tubes or flasks. Autoclave for 15 min at 121oC. If the upper portion of the is pink becouse of oxidaton, anaerobic mconditions can be restored (once only) by heating in a boiling water bath or by steam.

 

Quality specifications:    

Dehydrated medium:  homogeneous, cream-white fine powder.

Ready to use medium: straw-coloured, clear fluid.

 

Microbiological response:

 

Organism

Result

Clostridium perfringens ATCC13124

Growth

Staphylococcus aureus ATCC 25923

Growth

Bacteroides vulgatus ATCC 8482          

Growth

Candida albicans ATCC 10231

Growth

Bacillus subtilis ATCC 6633

Growth

 

 

 

 

Storage:  

Dehydrated medium should be stored between 10 to 25oC. Once opened, place the container in a dark, dry place. The dehydrated medium should not be used if there is any lump or the color has changed from the original.

 


فروشنده: آزمیران
گارانتی اصالت و سلامت کالا
پشتیبانی ۷ روز هفته ۲۴ ساعته
۰ تومان
(موجود نیست)
محصولات مرتبط

:

bsjylgrbucemmekrdchtzwooei55a3z5uyqe453oiustepcsqgja.mx-verification.google.com.

ابزار وبلاگ