تی سی بی اس TCBS ساخت شرکت میکرومدیا مجارستان 500 گرمی
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ویرایش

94.6.15

اعتبار قیمت

94.10.30

لطفا پس از پایان اعتبار قیمت با شماره تلفن 88920648 تماس حاصل فرمائید


Description of MM0183


 

                                          TCBS Agar

      (Thiosulphate Citrate Bile Salt Sucrose Agar)

 

 

Use and description:

T.C.B.S. is designed for the selective isolation of Vibrio cholerae and Vibrio parahaemolyticus. The high concentrations of thiosulphate and sodium citrate inhibit the growth of enterobacteria at least for 24 hours. Ox bile and sodium cholate slow the growth of enterococci and inhibit Gram-positive bacteria. Hydrogen sulphide production is visualized by blackening of media. All Vibrio are H2S-negative and sucrose fermenters Vibrio spp. appear as yellow colonies becouse of the acid production which turns the indicator from green to yellow.

 

Composition per liter:

Agar………………………………………..13.00 g

Peptones……………………………………15.00 g

Sodium citrate……………………………...10.00 g

Sodium thiosulphate……………………….10.00 g

Bacto bile……………………………………5.00 g

Sodium cholate……………………………...3.00 g

Sucrose…………………………………….20.00 g

Sodium chloride…………………………...10.00 g

Ferric citrate………………………………...1.00 g

Thymol blue………………………………...0.04 g

Bromothymol blue………………………….0.04 g

 

Final pH of the ready to use medium:   8.6 ± 0.2

 

Medium preparation:  

Add 87.0 grams of dehydrated culture medium to 1 liter of distilled water, mix until evenly dispersed. Heat with repeated stirring and boil for one minute. Poured plates can be prepared after the medium has been cooled to 45-50oC. DO NOT AUTOCLAVE THIS MEDIUM!

 

Quality specification:

Dehydrated medium: homogeneous, beige to greenish-beige, fine powder.

Ready to use medium: clear to trace hazy and forest green.

 

 

 

 

 

 

 

 

 

 

Microbiological response:

 

Organism

Result

Vibrio cholerae

Flat yellow colonies, 2-3 mm in diameter

Vibrio alginolyticus

Large yellow colonies           

Vibrio fluvialis, Vibrio vulnificus

Yellow or translucent colonies

Vibrio parahaemolyticus       

Colourless colonies with a green center

Pseudomonas, Aeromonas

Blue colonies

Enterobacteria or others

Tiny transparent colonies

Escherichia coli  ATCC 25922

Inhibited

Enterococcus faecalis ATCC 29212

Partially inhibited

Pseudomonas aeruginosa ATCC 10145

Partially inhibited

Vibrio cholerae ATCC 9459

Good, yellow colonies

Vibrio parahaemolyticus ATCC 17802

Good, colorless colonies with

blue-green center

 

Storage:  

Dehydrated medium should be stored between 10 to 25oC. Once opened, place the container in a dark, dry place. The dehydrated medium should not be used if there is any lump or the color has changed from the original.

 




فروشنده: آزمیران
گارانتی اصالت و سلامت کالا
پشتیبانی ۷ روز هفته ۲۴ ساعته
۱۴۵,۰۰۰ تومان
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