آزمیران
دستگاهای آزمایشگاهی
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شیشه آلات
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ائوزین یلو 100 گرمی کد A0822 ساخت شرکت اپلیکم آلمان
| Specification | ||||
|---|---|---|---|---|
| Synonym | Eosin Yellowish, 2',4',5',7'-Tetrabromofluorescein disodium salt | |||
| Transition interval | pH 0.0 - 3.0 (yellow - green fluorescence) | |||
| Formula | C20H6Br4Na2O5 | |||
| M | 691.88 g/mol | |||
| CAS-No.: | 17372-87-1 | |||
| HS-No.: | 32041200 | |||
| EC-No.: | 241-409-6 | |||
| Storage: | RT | |||
| LGK: | 10 - 13 | |||
| R: | 36 | |||
| S: | 22-26 | |||
 |
irritant | |||
| WGK: | 1 | |||
| Specification | ||||
| Assay (photometr.) | min. 90 % | |||
| λmax. | 514 - 520 nm | |||
| Loss on drying | max. 10 % | |||
| Literature |
|---|
|
(1) Selsted, M.E. & Becker III, H.W. (1986) Anal. Biochem. 155, 270-274 Eosin Y: A Reversible Stain for Detecting Electrophoretically Resolved Protein. |
| Comment |
|---|
|
The rapid staining of
proteins in low-pH, urea-containing polyacrylamide gels with eosin Y
reveal similar staining intensities to those seen with Coomassie® R-250.
High recoveries of total protein as well as enzymatic activity were
achieved (1). Protocol: Stain the gel for 30 seconds in 0.25 % eosin Y in 0.1 N NaOH. Rinse the gel for 10 seconds with distilled water. The eosin-stained proteins appeared as red-orange bands on a pale orange background. Backhround staining could be reduced by extending the destaining period to 30 minutes. Much longer destaining in water resulted in a loss of band intensity. Staining intensity could be stabilized indefinitely by storing the gel in 5 % acetic acid after 15 - 30 minutes of destaining in water (1). |
محصولات مرتبط
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۱۰,۸۰۰,۰۰۱تومان
۱۲,۶۰۰,۰۰۸تومان
۱۹,۸۰۰,۰۰۸تومان
۵,۸۵۰,۰۳۳تومان
