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KIT MINI SPIN BACTERIA GENOMICPREP 1 * 50 Tes (GE HEALTHCARE)
ویرایش
90.5.27
تاریخ اعتبار
90.7.27
لطفا پس از پایان اعتبار قیمت با شماره تلفن 88748085 تماس حاصل
فرمائید
جهت دریافت کاتالوگ لطفا روی عکس کلیک کنید
Technical Information
The illustra™ Bacteria genomicPrep Mini Spin Kit can be used to isolate genomic DNA from a wide range of bacterial strains such as DH5a, TOP10, JM109, Bacillus subtilis etc. The kit produces highly pure and intact genomic DNA with OD260/280 values of 1.75-1.85. You can use different elution volumes to isolate genomic DNA at concentrations suitable for most downstream applications including restriction enzyme digestion, RT-PCR, and long PCR. The method uses a lysis solution in combination with proteinase K to gently release genomic DNA into solution from bacteria cells. The DNA is deproteinated in the extraction solution and the genomic DNA is then bound onto a silica column in the presence of chaotropic solution. Contaminants are removed during wash steps and the purifed DNA is eluted with prewarmed TE solution.
The kit contains illustra bacteria genomicPrep Mini spin columns prepacked with novel silica membrane, lyophilized proteinase K powder, lysis solutions, wash and elution buffers, 2 ml microcentrifuge collection tubes, a full booklet with a detachable, quick reference protocol card.
Restriction enzyme digestion of gDNA purified using illustra bacteria genomicPrep Mini Spin Kit and QIAamp DNA Mini Kit. Purified samples containing 250 ng of gDNA were used for digestion reactions with 20 to 80 units of enzyme at either 37°C for 18 hours. The results were visualized on 0.8% agarose gel. U = undigested (no enzyme) gDNA; B = BamHI; E = EcoRI; H = HindIII; M = 1 kb molecular weight marker.
| TECHNICAL SPECIFICATIONS | |
| bacteria Mini (1 × 109 to 4 × 109 cells) | |
| Yield | 4 to 12 μg |
| Purity | 1.7 to 1.9 |
| Total time of prep* (gram negative) | 40 min |
| Hands-on time* | 15 min |
| Total time of prep* (gram positive) | 55 min |
| Hands-on time* | 15 min |
| Size | > 20 kb |
| *includes testing on a wide range of strains
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References
- Human Genome Resources [Online] http://www.ncbi.nlm.nih.gov/genome/guide/human
- Sambrook, J. and Russell, D. W. Molecular Cloning, A Laboratory Manual, chapter 6 (2001).
- Vogelstein, B. and Gillespie, D. Proc. Natl. Acad. Sci. USA 76, 615 (1979).
فروشنده: فروشگاه
گارانتی اصالت و سلامت کالا
پشتیبانی ۷ روز هفته ۲۴ ساعته
۴۶۰,۰۰۰ تومان
محصولات مرتبط
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۱۰,۵۰۰,۰۰۱تومان
۱۶,۵۰۰,۰۰۱تومان
۴,۸۷۵,۰۲۴تومان
