آزمیران
دستگاهای آزمایشگاهی
مواد شیمیایی
شیشه آلات
محیط کشتهای میکروبی
فیلتر یا کاغذ صافی
ملزومات
سکوبندی
مقالات و توضیحات
جستجوهای مرتبط
KIT MIDI FLOW ILLUSTRA BLOOD GENOMICPREP 1 * 25 Tes (GE HEALTHCARE)
ویرایش
90.5.27
تاریخ اعتبار
90.7.27
لطفا پس از پایان اعتبار قیمت با شماره تلفن 88748085 تماس حاصل
فرمائید
جهت دریافت کاتالوگ لطفا روی عکس کلیک کنید
Technical Information
Lanes 1, 3, 5 represent the samples digested with EcoRI for 2 h.
Lanes 2, 4, 6 represent genomic DNA used in the digestion experiments.
The illustra™ blood genomicPrep Midi Flow Kit can be used to purify
genomic DNA from human whole blood, buffy coat, and animal blood such as
horse, sheep, rat, mouse, guinea pig, and chicken whole blood. The kit
provides yields of up to 250 μg of high-quality genomic DNA. The kit is
robust, highly reproducible, and versatile allowing you to choose either
gravity or spin methods with input sample volumes of 1 to 8 ml. The
genomic DNA isolated with the illustra blood genomicPrep Midi Flow Kit
is suitable for most biological applications including PCR, real-time
PCR, restriction enzyme digest, and sequencing. The kit uses a simple
and efficient three-step process involving a two-step lysis procedure
at ambient temperature, anion-exchange column chromatography, and a
highly reproducible desalting process. The kit contains fast-flow
genomic 250 columns, lyophilized proteinase K powder, lysis solutions,
load and elution buffers, NAP-25 desalting column, spin column adapters,
a full protocol
booklet, and a detachable, quick reference protocol card.
Pulse-field gel electrophoresis showing high-quality genomic DNA isolated from human whole blood using the illustra blood genomicPrep Midi Flow Kit and a QIAGEN blood & cell culture midi kit. Lanes 1 and 2 are lambda and low-range markers, Lanes 3 to 14 represent genomic DNA purified with the illustra blood genomicPrep Midi Flow Kit, and Lanes 15 to 19 represent genomic DNA purified with a Qiagen blood & cell culture midi kit.
TECHNICAL SPECIFICATIONS | |
blood Midi (1 to 8 ml; 5 ml optimum) | |
Yield | 85 to 120 μg |
Purity | 1.7 to 1.9 |
Total time of prep* | 2 h |
Hands-on time* | 45 min |
Size | > 50 kb |
*including lysis, purification & desalting |
فروشنده: فروشگاه
گارانتی اصالت و سلامت کالا
پشتیبانی ۷ روز هفته ۲۴ ساعته
۸۲۰,۰۰۰ تومان