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مقالات و توضیحات
جستجوهای مرتبط
KIT MIDI FLOW ILLUSTRA BLOOD GENOMICPREP 1 * 50 Tes (GE HEALTHCARE)
ویرایش
90.5.27
تاریخ اعتبار
90.7.27
لطفا پس از پایان اعتبار قیمت با شماره تلفن 88748085 تماس حاصل
فرمائید
جهت دریافت کاتالوگ لطفا روی عکس کلیک کنید
Technical Information
Real-time PCR amplification from genomic DNA extracted from K3 EDTA-treated whole blood using the illustra blood genomicPrep Mini Spin Kit. The eluted product (3 μl ) and various amounts (0.1, 1, 10, and 100 ng) of control DNA were amplified using chromosome-specific primers in a real-time PCR assay on an ABI 7900. Replicates were n = 3.
The purification process involves minimal shearing resulting in the
production of 5 to 10 μg of good quality, intact genomic DNA from a
200 μl sample. Input sample volumes can vary from 50 μl to 1 ml. The
method uses a simple genomic DNA purification protocol that uses
chaotropic agents to extract DNA from blood cells, denature protein
components, and promote the selective binding of DNA to a column-based,
novel silica-membrane. The elution volume can be modified as necessary
to obtain the appropriate genomic DNA concentration for most downstream
molecular biological applications. The kit contains spin columns
prepacked with a novel silica membrane, lyophilized proteinase K powder,
lysis solution, wash and elution buffers, microcentrifuge collection
tubes, a full protocol booklet, and a detachable, quick reference
protocol card.
Digestion of purified genomic DNA with restriction enzymes. Purified genomic DNA from the blood genomicPrep Mini Spin Kit was digested with AluI, EcoRI, HindIII, and BamHI restriction enzymes.
Kit compatibility across multiple anticoagulants. Isolation of genomic DNA was carried out from 200 μl of human (Hu) whole blood treated with K3-EDTA (EDTA), sodium citrate (Cit) and sodium heparin (Hep). The plot shows mean values for yield and purity with standard deviations.
| TECHNICAL SPECIFICATIONS | |
| Yield | 4 to 6 μg/200 μl whole blood |
| Purity | > 1.7 to 1.6 |
| Total time of prep* | less than 20 min |
| Hands-on time* | less than 5 min |
| Size | > 20 kbp |
| *including lysis, purification & desalting
|
|
References
- Sambrook, J., Fritsch, E.F and Maniatis, T. Molecular cloning: A laboratory Manual, Cold Spring Harbor laboratory, 2nd eds., (1989).
- Vogelstein, B. and Gillespie, D. Proc. Natl. Acad. Sci. USA 76, 615 (1979).
- Marko, M.A., Chipperfield, R. and Birnboim, H.C. Anal. Biochem. 121, 382 (1982).
- Voo, K.S. and Jacobsen B.M., Biotechniques 24, 240-243 (1998).
- Hilz, H. et al. Eur. J. Biochem. 56, 103-108 (1975).
- Ausubel, F.M. et al., eds., Current Protocols in Molecular Biology 1, 1.68 (1991).
- Melzak, K. A. et al., J. Coll. Interf. Sci. 181, 635-644 (1996).
فروشنده: فروشگاه
گارانتی اصالت و سلامت کالا
پشتیبانی ۷ روز هفته ۲۴ ساعته
۴۰۰,۰۰۰ تومان
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