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A-003-M Sigma-Aldrich Poly-D-Lysine solution, 1.0 mg/mL 20ml
زمان تحویل 8 هفته
Properties
Quality Level 100
form liquid
mfr. no. Specialty Media
application(s) cell analysis: suitable (cell culture)
input sample type induced pluripotent stem cell(s)
sample type mesenchymal stem cell(s)
sample type neural stem cell(s)
sample type epithelial cells
sample type hematopoietic stem cell(s)
sample type: human embryonic stem cell(s)
sample type pancreatic stem cell(s)
Description
General description
Poly-D-Lysine promotes the adhesion of tissues/sections to the culture vessel. The polycationic property of the molecule allows interaction with anionic sites on the tissue/cells promoting effective attachment to the growing surface. The molecular weight of Poly-D-Lysine is >300,000 Da.
Application
The level of coating will vary for different species and types of cells. In general final coating concentrations from 3-10μg/cm(2) of surface area will typically supply sufficent coatings for most cell types.
Surface area of plates: (pi)(Radius squared) in cm(2)
In partical terms working dilutions of 50μg/mL to 100μg/mL are usually adequate.
Dilute poly-D-lysine in PBS, water, or 0.1M borate buffer {prepared by adding 1.24 g boric acid and 1.9 g sodium tetraborate in 400 ml water, pH 8.5} to final working concentration.
Plates or flasks are coated for 3 hours to overnight, and up to 3 days if stored at 4C. Best results are usually with just over night coatings.
Remove poly-d-lysine solution and rinse with sterile PBS or water 1-2X. Do not scrape the bottom of the coated plates or flasks.
Use plates immediately or store with PBS for up to 5 days.
Poly-D-lysine coating methods will vary slightly from laboratory to laboratory, please use the method as a general guide.
Physical form
Liquid. 20ml in sterile water.
Storage and Stability
Store at -20C for upto 18 months.
Safety Information
Safety Information for this product is unavailable at this time.
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