آنتی بادی Anti-Mu Opioid Receptor antibody ab10275
آنتی بادی  Anti-Mu Opioid Receptor antibody ab10275

 

اعتبار قیمت 95.7.1

 

لطفا پس از پایان اعتبار قیمت با تلفن 88920648 تماس حاصل فرمائید 

 

Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab10275 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

ApplicationAbreviewsNotes
ICC/IF1/100 - 1/2000.
WB1/500 - 1/1000. Detects a band of approximately 49-65 kDa (predicted molecular weight: 45 kDa).Can be blocked with Rat Mu Opioid Receptor peptide (ab46988).

Approximately 49 -65 kDa depending on the tissue lysate. Can be blocked withab46988.

IHC-Fr1/100 - 1/2000. We recommend incubating for 18-24 hours at 4C.
ICC1/100 - 1/2000.
IHC-FoFr1/100 - 1/2000. We recommend incubating for 18-24 hours at 4C.

Target

Anti-Mu Opioid Receptor antibody images

  • Ab10275 (1:2500) staining of Mu opioid receptor by IHC.
  • ICC/IF image of ab10275 stained SHSY5Y cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab10275, 1 in 200 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • فروشنده: واردکننده
    گارانتی اصالت و سلامت کالا
    پشتیبانی ۷ روز هفته ۲۴ ساعته
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فروش مواد شیمیایی فقط جهت مراکز دولتی، شرکتها، پژوهشگاهها، محققین و اساتید دانشگاه انجام میشود لطفا درخواست خود را به شماره واتس آپ یا تلگرام یا ایتا 09365036989 ارسال کنید