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Donkey Anti-Sheep IgG H&L (Alexa Fluor® 488) ab150177 500 µg
Donkey Anti-Sheep IgG H&L (Alexa Fluor® 488) ab150177 500 µg
اعتبار قیمت 95.7.1
لطفا پس از پایان اعتبار قیمت با تلفن 88920648 تماس حاصل فرمائید
Overview
- Product nameDonkey Anti-Sheep IgG H&L (Alexa Fluor® 488)
See all IgG secondary antibodies - DescriptionDonkey polyclonal Secondary Antibody to Sheep IgG - H&L (Alexa Fluor® 488)
- Target speciesSheep
- Tested applicationsIHC-Fr, ICC/IF, ELISA, IHC-P, Flow Cytmore details
- ConjugationAlexa Fluor® 488. Ex: 495nm, Em: 519nm
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
- Storage bufferPreservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol, 1% BSA -
Concentration 200 µg at 2 mg/ml 500 µg at 2 mg/ml
- PurityImmunogen affinity purified
- Purification notesThis antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
- ClonalityPolyclonal
- IsotypeIgG
- General notes
The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to use products containing Alexa Fluor® dyes for purposes other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.
- Research areas
Applications
Our Abpromise guarantee covers the use of ab150177 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr | Use at an assay dependent concentration. | |
| ICC/IF | 1/200 - 1/1000. | |
| ELISA | Use at an assay dependent concentration. | |
| IHC-P | Use at an assay dependent concentration. | |
| Flow Cyt | 1/2000. |
Donkey Anti-Sheep IgG H&L (Alexa Fluor® 488) images
-
Flow Cytometry - Donkey Anti-Sheep IgG H&L (Alexa Fluor® 488) (ab150177)Overlay histogram showing HeLa cells stained with ab21976 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal donkey serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab21976, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody Donkey anti-sheep IgG H&L (Alexa Fluor® 488) (ab150177) was used at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was sheep IgG (ab37385, 0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
-
Immunocytochemistry/ Immunofluorescence - Donkey polyclonal Secondary Antibody to Sheep IgG - H&L (Alexa Fluor® 488) (ab150177)ICC/IF image of ab7291 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7291, 5µg/ml) overnight at +4°C. Ab6710, sheep anti-mouse IgG, was then added as a secondary bridging antibody, at 1/250 dilution for 1h. Ab150177 Alexa Fluor® 488 donkey anti-sheep IgG (H+L) (green) was then used at 2µg/ml for 1h as a tertiary antibody. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
Immunocytochemistry/ Immunofluorescence - Donkey Anti-Sheep IgG H&L (Alexa Fluor® 488) (ab150177)HeLa cells showing negative staining by ICC/IF using only secondary antibody. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. ab150177 Alexa Fluor® 488 donkey anti-sheep IgG (H+L) (green) was used at 2µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
Alexa Fluor® - Donkey Anti-Sheep IgG H&L (Alexa Fluor® 488) (ab150177)
References for Donkey Anti-Sheep IgG H&L (Alexa Fluor® 488) (ab150177)
ab150177
has not yet been referenced specifically in any publications.
فروشنده: واردکننده
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