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ملزومات
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مقالات و توضیحات
جستجوهای مرتبط
AB SPINTRAP 50 × 100 µL 1 * 50 ite (GE HEALTHCARE)
ویرایش
90.5.27
تاریخ اعتبار
90.7.27
لطفا پس از پایان اعتبار قیمت با شماره تلفن 88748085 تماس حاصل
فرمائید
جهت دریافت کاتالوگ لطفا روی عکس کلیک کنید
Technical Information
Column: Ab SpinTrap, prepacked with 100 μl Protein G Sepharose High Performance
Equilibration: 600 μl 20 mM sodium phosphate, pH 7.0
Sample: 600 μl undiluted serum
Incubation: 4 min
Wash: 2 × 600 μl 20 mM sodium phosphate, pH 7.0
Elution: 2 × 400 μl 0.1 M glycine-HCl, pH 2.7
Lane
1 Low molecular weight markers
2 Eluted pool (diluted 1:5)
3 Start material (diluted 1:50)
Ab SpinTrap™ is designed for efficient, small-scale purification of monoclonal and polyclonal antibodies from serum and cell culture supernatants. In addition, a protocol for immunoprecipitation is included.
The spin columns are also suitable for use in antibody screening experiments.
Ab SpinTrap™ columns are used together with a standard microcentrifuge and one purification run is performed in less than 20 min.
Ab SpinTrap™ contains Protein G Sepharose™ High Performance, which has high protein binding capacity and is compatible with all commonly buffers used in antibody purification with immobilized Protein G media.
For optimal performance in antibody purification, use Ab SpinTrap™ together with buffers prepared using Ab Buffer Kit.
| TECHNICAL SPECIFICATIONS | |
| Medium | Protein G Sepharose™ High Performance |
| Average particle size | 34 μm |
| Bed volume | 100 μl |
| Max sample volume | 600 μl |
| Binding capacity* | > 1 mg IgG/column |
| Compatibility during use | Stable in all commonly used buffers within antibody purification |
| pH stability | |
| Short term (2h) | 2-9† |
| Storage | 20% ethanol |
| Storage temperature | 4°C to 8°C |
| Column material | Polypropylene barrel and polyethylene frits |
| Column | Ab SpinTrap, prepacked with 100u?μl Protein G Sepharose High Performance |
| Equilibration | 600 μl 20 mM sodium phosphate, pH 7.0 |
| Sample | 600 μl undiluted serum |
| Incubation | 4 min |
| Wash | 2 × 600 μl 20 mM sodium phosphate, pH 7.0 |
| Elution | 2 × 400 μl 0.1 M glycine-HCl, pH 2.7 |
| Column material | Polypropylene barrel and polyethylene frits |
| * The binding capacity has been determined with human polyclonal IgG. † The range given is estimated based on our knowledge and experience. A pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH. | |
فروشنده: فروشگاه
گارانتی اصالت و سلامت کالا
پشتیبانی ۷ روز هفته ۲۴ ساعته
۱,۱۰۰,۰۰۰ تومان
محصولات مرتبط
:
۱۰,۵۰۰,۰۰۱تومان
۱۶,۵۰۰,۰۰۱تومان
۴,۸۷۵,۰۲۴تومان
