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جستجوهای مرتبط
KIT MINI SPIN ILLUSTRA BLOOD GENOMICPREP 1 * 250 Tests (GE HEALTHCARE)
ویرایش
90.5.26
اعتبار قیمت
90.8.26
لطفا پس از پایان اعتبار قیمت با شماره تلفن 88748085 تماس حاصل
فرمائید
جهت دریافت کاتالوگ لطفا روی عکس کلیک کنید
Membrane Protein Purification Kit for histidine-tagged membrane proteins allows rapid and easy detergent screening for simultaneous evaluation of solubilization and purification efficiency.
Membrane Protein Purification Kit is designed for efficient
detergent screening of histidine-tagged membrane proteins. Small
aliquots of cell membranes are solubilized in different detergents
followed by rapid purification using His Mag Sepharose™ Ni. The
purification step is performed directly after solubilization using the
same detergent. Analysis and evaluation can be performed by a number of
methods such as Western blot, gel filtration, or light scattering.
Membrane Protein Purification Kit is sufficient for two complete
screening experiments according to the included protocol.
The magnetic bead format is excellent for small-scale experiments.
The high density of the beads allows rapid capture by magnetic devices,
while the visibility of the beads ensures reliable collection of the
bound histidine-tagged membrane proteins in the screening and
purification procedure. Purification of target membrane proteins for a
number of analytical and/or other downstream applications can be
achieved within a few hours. For convenient purification of larger
amounts of membrane proteins, use prepacked HisTrap™ HP columns.
| TECHNICAL SPECIFICATIONS | ||||
| Kit Contains* | Amount supplied | CMC† (%) | CMC† (mM) | Detergent class |
| His Mag Sepharose Ni | 3 x 1 ml vial | |||
| n-Dodecyl-b-D-maltoside (DDM) | 10% (w/v) aqueous solution, 1 ml | 0.009 | 0.17 | Non-ionic |
| n-Decyl-b-D-maltoside (DM) | 10% (w/v) aqueous solution, 1 ml | 0.09 | 1.8 | Non-ionic |
| Lauryldimethylamine-N-oxide (LDAO) | 10% (w/v) aqueous solution, 1 ml | 0.02 | 1-2 | Zwitter-ionic |
| n-Dodecylphosphocholine (FOS12) | 10% (w/v) aqueous solution, 1 ml | 0.05 | 1.5 | Non-ionic |
| Dodecyl octaethyleneglycol ether (C12E8) | 10% (w/v) aqueous solution, 1 ml | 0.005 | 0.09 | Non-ionic |
| Cyclohexyl-1-pentyl-b-D-maltoside (Cymal ™5) | 10% (w/v) aqueous solution, 1 ml | 0.12 | 2-5 | Zwitter-ionic |
| n-Octyl-b-D-glucoside (OG) | 20% (w/v) aqueous solution, 1 ml | 0.53 | 18-20 | Non-ionic |
| Phosphate buffer (stock), pH 7.4 (160 mM sodium phosphate, 4 M NaCl) | 100 ml | |||
| 2 M imidazole pH 7.4 | 100 ml | |||
*Analytical grade, >99% by HPLC analysis; all detergents are from Anatrace™ Maumee, Ohio, USA (Affymetrix).
†Approximate critical micelle concentrations (CMC) in water at 20°C.
†Approximate critical micelle concentrations (CMC) in water at 20°C.
| TECHNICAL SPECIFICATIONS | |
| His Mag Sepharose Ni | |
| Matrix | Highly cross-linked spherical agarose (Sepharose) including magnetite |
| Metal ion capacity | ~21 μmol Ni2+/ml medium |
| Binding capacity | approximately 50 mg histidine-tagged protein/ml medium (~500 μg/purification run) |
| Particle size | 37 to 100 μm |
| Storage solution | 20% ethanol, 5% medium slurry |
| Entire kit | |
| Working temperature | Ambient |
| Storage temperature | +2°C to +8°C |
فروشنده: آزمیران
گارانتی اصالت و سلامت کالا
پشتیبانی ۷ روز هفته ۲۴ ساعته
۲,۲۰۰,۰۰۰ تومان
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