Caspase 8 Assay Kit (Colorimetric) ab39700
Caspase 8 Assay Kit (Colorimetric) ab39700


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Overview

  • Product nameCaspase 8 Assay Kit (Colorimetric)
    See all Caspase 8 kits
  • Detection methodColorimetric
  • Tests
    100 x 1 assay
  • Sample type
    Cell Lysate
  • Assay typeEnzyme activity
  • Assay time
    2h 00m
  • Product overview

    Caspase 8 Assay Kit (Colorimetric) (ab39700) provides a simple and convenient means for assaying the activity of caspases that recognize the sequence IETD. The assay is based on spectrophotometric detection of the chromophore p-nitroanilide (p-NA) after cleavage from the labeled substrate IETD-p-NA. The p-NA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400 or 405 nm. Comparison of the absorbance of p-NA from a treated sample with an untreated control allows determination of the fold increase in Caspase 3 activity.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells.

  • Tested applicationsFunctional Studiesmore details

Properties

  • RelevanceCaspases are cysteine proteases, expressed as inactive precursors, that mediate apoptosis by proteolysis of specific substrates. Caspases have the ability to cleave after aspartic acid residues. There are two classes of caspases involved in apoptosis; initiators (activation by receptor cluster) and effectors (activation by mitochondrial permeability transition). Proapoptotic signals autocatalytically activate initiator caspases, such as Caspase 8 and Caspase 9. Activated initiator caspases then process effector caspases, such as Caspase 3 and Caspase 7, which in turn cause cell collapse.
  • Cellular localizationCytoplasmic
  • Alternative names
    • Apoptotic cysteine protease
    • CAP4
    • CASP8
    see all

Applications

Our Abpromise guarantee covers the use of ab39700 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

ApplicationAbreviewsNotes
Functional StudiesUse at an assay dependent concentration.

Caspase 8 Assay Kit (Colorimetric) images



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فروش مواد شیمیایی فقط جهت مراکز دولتی، شرکتها، پژوهشگاهها، محققین و اساتید دانشگاه انجام میشود لطفا درخواست خود را به شماره واتس آپ یا تلگرام یا ایتا 09365036989 ارسال کنید