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Cyclic GMP Direct ELISA Kit ab133027 1 x 96 tests
Cyclic GMP Direct ELISA Kit ab133027 1 x 96 tests
اعتبار قیمت 95.7.1
لطفا پس از پایان اعتبار قیمت با تلفن 88920648 تماس حاصل فرمائید
Overview
- Product nameCyclic GMP Direct ELISA Kit
See all cGMP kits - Detection methodColorimetric
- Sample typeTissue, Cell Lysate
- Assay typeCompetitive
- Assay durationMultiple steps standard assay
- Product overview
Abcam’s Cyclic GMP (cGMP) Direct in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Cyclic GMP in tissue culture media treated with 0.1 M HCl.
A goat anti-rabbit IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated-cGMP antigen and a polyclonal rabbit antibody specific to cGMP. After incubation the excess reagents are washed away. pNpp substrate is added and after a short incubation the enzyme reaction is stopped and the yellow color generated is read at 405 nm. The intensity of the yellow coloration is inversely proportional to the amount of cGMP captured in the plate.
- Notes
Guanosine 3’, 5’-cyclic monophosphate (cyclic GMP; cGMP) has been shown to be present at levels typically 10-100 fold lower than cAMP in most tissues and is formed by the action of the enzyme guanylate cyclase on GTP. Some hormones, such as acetylcholine, insulin and oxytocin, as well as certain other chemicals like serotonin and histamine cause an increase in cGMP levels. Stimulators of guanylate cyclase such as the vasodilators nitroprusside, nitroglycerin, sodium nitrate and nitric oxide (NO) also increase cGMP levels. NO can be synthesized from L-arginine and diffuse through cell membranes. The interaction of NO with guanylate cyclase allows cGMP to act as a third messenger in some cells. Bioactive peptides like atrial natriuretic peptide (ANP) also increase cGMP concentrations.
Cross Reactivity
Compound % Cross Reactivity cGMP 100 GMP <0.001 GTP <0.001 cAMP <0.001 AMP <0.001 ATP <0.001 cUMP <0.001 CTP <0.001 - Tested applicationsCompetitive ELISAmore details
- PlatformMicroplate
Properties
- Storage instructionsPlease refer to protocols.
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Components 1 x 96 tests 0.1M HCl 1 x 27ml 20X Wash Buffer Concentrate 1 x 27ml Acetylation kit - Acetic Anhydride 1 x 1ml Acetylation kit - Triethylamine 1 x 2ml Cyclic GMP Complete Antibody 1 x 5ml Cyclic GMP Complete Standard 1 x 500µl Cyclic GMP Direct Alkaline Phosphatase Conjugate 1 x 5ml Goat anti-rabbit IgG Microplate (12 x 8 wells) 1 unit Neutralizing Reagent 1 x 5ml Plate Sealer 1 unit pNpp Substrate 1 x 20ml Stop Solution 1 x 5ml - Research areas
- RelevanceCyclic guanosine monophosphate (cGMP) serves as a second messenger in a manner similar to that observed with cAMP. Peptide hormones, such as the natriuretic factors, activate receptors that are associated with membrane-bound guanylate cyclase (GC). Receptor activation of GC leads to the conversion of GTP to cGMP. Nitric oxide (NO) also stimulates cGMP production by activating soluble GC, perhaps by binding to the heme moiety of the enzyme. Similar to cAMP, cGMP mediates most of its intracellular effects through the activation of specific cGMP dependent protein kinases (PKG).
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Alternative names
- Cyclic GMP
- Cyclic guanosine monophosphate
- Guanosine 3 5 Cyclic Monophosphate
Applications
Our Abpromise guarantee covers the use of ab133027 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Competitive ELISA | Use at an assay dependent concentration. |
Cyclic GMP Direct ELISA Kit images
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Typical Standard CurveRepresentative Standard Curve using ab133027.
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Typical Standard CurveRepresentative Standard Curve using ab133027
Protocols
References for Cyclic GMP Direct ELISA Kit (ab133027)
ab133027
has not yet been referenced specifically in any publications.
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