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Caspase 9 Assay Kit (Fluorometric) (ab65607) 100 tests
اعتبار قیمت 95.10.1
Overview
- Product nameCaspase 9 Assay Kit (Fluorometric)
See all Caspase 9 kits - Tests100 x 1 assay
- Sample typeTissue Extracts, Cell Lysate
- Assay typeEnzyme activity
- Assay time2h 00m
- Product overview
Abcam's Caspase 9 Assay Kit (Fluorometric) provides a simple and convenient means for assaying the activity of caspases that recognize the sequence LEHD. The assay is based on detection of cleavage of substrate LEHD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). LEHD-AFC emits blue light (?max = 400 nm); upon cleavage of the substrate by caspase-9 or related caspases, free AFC emits a yellow-green fluorescence (?max = 505 nm), which can be quantified using a fluorometer or fluorecence microtiter plate reader.
Visit our FAQs page for tips and troubleshooting. - Notes
Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells.
- Tested applicationsFunctional Studiesmore details
Properties
- Storage instructionsStore at -20°C. Please refer to protocols.
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Components 100 tests 2X Reaction Buffer 4 x 2ml Cell Lysis Buffer 1 x 100ml DTT 1 x 400µl LEHD-AFC (1mM) 1 x 500µl - Research areas
- RelevanceCaspases are cysteine proteases, expressed as inactive precursors, that mediate apoptosis by proteolysis of specific substrates. Caspases have the ability to cleave after aspartic acid residues. There are two classes of caspases involved in apoptosis; initiators (activation by receptor cluster) and effectors (activation by mitochondrial permeability transition). Proapoptotic signals autocatalytically activate initiator caspases, such as Caspase 8 and Caspase 9. Activated initiator caspases then process effector caspases, such as Caspase 3 and Caspase 7, which in turn cause cell collapse. Caspase 9 (also known as ICE like apoptotic protease 6 (ICE LAP6), apoptotic protease Mch6, and apoptotic protease activating factor 3 (Apaf3)) is a member of the peptidase family C14 that contains a CARD domain. It is active as a heterotetramer and has been reported to have two isoforms. ProCaspase 9 is approximately 47 kD. It is present in the cytosol and, upon activation, translocates to the mitochondria. Caspase 9 is involved in the caspase activation cascade responsible for apoptosis execution and cleaves/activates Caspase 3 and Caspase 6. It becomes activated when recruited to the Apaf1/cytochrome c complex.
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Alternative names
- APAF3
- CASP9
- Caspase 9
see all
Associated products
- Related Products
Applications
Our Abpromise guarantee covers the use of ab65607 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Functional Studies | Use at an assay dependent dilution. |
Caspase 9 Assay Kit (Fluorometric) images
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Functional Assay: ab65607 Caspase 9 assay kit (Fluorometric)
Protocols
References for Caspase 9 Assay Kit (Fluorometric) (ab65607)
This product has been referenced in:
- Lee YJ et al. Apoptotic cell instillation after bleomycin attenuates lung injury through hepatocyte growth factor induction. Eur Respir J 40:424-35 (2012). Read more (PubMed: 22441736) »
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