آزمیران
دستگاهای آزمایشگاهی
مواد شیمیایی
شیشه آلات
محیط کشتهای میکروبی
فیلتر یا کاغذ صافی
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سکوبندی
مقالات و توضیحات
جستجوهای مرتبط
اوره آگار بیس ( بدون اوره ) 500 گرمی ساخت شرکت میکرومدیا مجارستان
Description of MM0066
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Urea Agar Base
Use and description: Media used for the detection of Proteus species, based on urease activity and for the identification of other members of the Enterobacteriaceae based on urease activity. Urease-positive bacterias produce ammonia, which increase the pH, so couse colour-changing of phenol red from orange to purple.
Composition per liter: Agar………………………………………15.200 g Sodium chloride…………………………...5.000 g Disodium hydrogen phosphate…………….1.200 g Peptone…………………………………….1.000 g Glucose…………………………………….1.000 g Potassium dihydrogen phosphate………….0.800 g Phenol red………………………………….0.012 g
Final pH of the ready to use medium: 6.8 ± 0.2
Medium preparation: Add 24.7 grams of dehydrated culture medium to 950 mL of distilled water. Bring to the boil to dissolve completely. Autoclave at 121.0oC for 15 minutes. Cool the agar base to 45 to 50oC and aseptically add the 50 mL filter-sterilised Urea solution. Mix thoroughly and dispense into sterile tubes.
Quality specification: Dehydrated medium: homogeneous, creamy, fine powder. Ready to use medium: yellow/pale pink, translucent.
Microbiological response:
Storage: Dehydrated medium should be stored between 10 to 25oC. Once opened, place the container in a dark, dry place. The dehydrated medium should not be used if there is any lump or the color has changed from the original. |
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