Description of MM0039
Tryptone Water Broth
Use and description: This medium is used for testing of indol prioduction by bacteria that has the ability to utilise tryptophane.
Composition per liter: Tryptone………………………………………..10.0 g Sodium chloride………………………………..5.0 g
Final pH of the ready to use medium: 7.5 ± 0.2
Medium preparation: Add 15.0 grams of dehydrated culture medium to 1 liter of distilled water. Heat with repeated stirring and boil for one minute to dissolve completely. Distribute and autoclave at 121.0oC for 15 minutes.
Quality specifications: Dehydrated medium: homogeneous, light beige fine powder. Ready to use medium: clear and pale yellow.
Microbiological response:
Storage: Dehydrated medium should be stored between 10 to 25oC. Once opened, place the container in a dark, dry place. The dehydrated medium should not be used if there is any lump or the color has changed from the original. |
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