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پپتون سویا ( سوی پپتون ) ساخت شرکت میکرومدیا مجارستان 500 گرمی
Description of MM0337
Raka Ray agar
Use and description: Medium used for the isolation of lactic acid bacteria in beer and brewing processes. It enables monitoring in-process beer quickly for numerous organisms.
Composition per liter:
Yeast extract………………………………….......5.00 g Tryptone………………………………………. 20.00 g Liver extract……………………………………...1.00 g Maltose………………………………………….10.00 g Fructose…………………………………………..5.00 g Glucose…………………………………………...5.00 g Betain HCl………………………………………..2.00 g Diammonium-H-citrate…………………………..2.00 g Potassium aspartame……………………………..2.50 g Potassium glutamate……………………………...2.50 g Magnesium sulphate……………………………...0.98 g Manganese sulphate………………………………0.42 g Potassium phosphate……………………………...2.00 g N-acetyl-glucosamine…………………………….0.50 g Agar……………………………………………..15.00 g
Final pH of the ready to use medium: 5.4 ± 0.2
Medium preparation: Add 73.9 grams of dehydrated culture medium to 1 liter of distilled water and add 10 ml Tween 80 to it. Mix until evenly dispersed with stirring. Boil for one minute. Autoclave for 15 min at 121 °C and to increase selectivity add aseptically 3 mg cycloheximide and 3 g phenylethanol and pour into tubes or petri dishes.
Quality specifications: Dehydrated medium: homogeneous, beige fine powder. Ready to use medium: clear to sligthly haisy, amber colored agar.
Microbiological response after 24-48 hours at 27-30 °C, under anaerobic condition:
Organism Result
Storage: Dehydrated medium should be stored between 10 to 25.0oC. Once opened, place the container in a dark, dry place. The dehydrated medium should not be used if there is any lump or the color has changed from the original. |
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