آزمیران
دستگاهای آزمایشگاهی
مواد شیمیایی
شیشه آلات
محیط کشتهای میکروبی
فیلتر یا کاغذ صافی
ملزومات
سکوبندی
مقالات و توضیحات
جستجوهای مرتبط
بایل اسکولین آگار بیس 500 گرمی میکرومدیا مجارستان
Description of MM0136
|
Bile Esculin Agar
Use and description: For the isolation and presumptive identification of enterococci (Group D streptococci). Enterococci has the ability to hydrolyse esculin. The final product, dihydroxy-6,7 coumarin, combines with iron ions to produce a dark to black-coloured compound that diffuses into the medium (black halo arround the colonies). Bile renders the medium inhibitor with regard to other bacteria.
Composition per liter: Bile salts………………………….20.0 g Agar………………………………13.0 g Peptone…………………………….5.0 g Meat extract………………………..3.0 g Ferric citrate……………………….0.5 g Esculin……………………………..1.0 g
Final pH of the ready to use medium: 6,9 ± 0.2
Medium preparation: Add 42.5 grams of dehydrated culture medium to 1 liter of distilled water. Heat with repeated stirring to dissolve completely. Sterilise by autoclaving at 121oC for 15 minutes.
Quality specifications: Dehydrated medium: homogeneous, beige fine powder. Ready to use medium: dark amber with blueish cast and opalescent.
Microbiological response:
Storage: Dehydrated medium should be stored between 10 to 25oC. Once opened, place the container in a dark, dry place. The dehydrated medium should not be used if there is any lump or the color has changed from the original. |
محصولات مرتبط
:
