Description of MM0064
Plate Count Agar
Use and description: This medium recommended by USP and APHA is used to count aerobic bacteria in water, milk and other food products.
Composition per liter: Yeast extract………………………………..2.5 g Tryptone……………………………………5.0 g Glucose…………………………………….1.0 g Agar………………………………………12.0 g
Final pH of the ready to use medium: 7.0 ± 0.2
Medium preparation: Add 23.5 grams of dehydrated culture medium to 1 liter of distilled water. Bring to the boil with frequent stirring to dissolve. Dispense into tubes and sterilise by autoclaving for 15 min at 121oC. Cool to 44-46 oC for not more than 3 hours prior to use. Incubate at 32-35 oC for 48 hours.
Quality specifications: Dehydrated medium: homogeneous, cream coloured fine powder. Ready to use medium: light amber agar, very slightly opalescent.
Microbiological response:
Storage: Dehydrated medium should be stored between 10 to 25oC. Once opened, place the container in a dark, dry place. The dehydrated medium should not be used if there is any lump or if the color has changed from the original. |
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