ای ام بی آگار EMB ساخت شرکت میکرومدیا مجارستان 500 گرمی
Description of MM0110

 

                             EMB agar (Eosin Methylene Blue

Agar, Levine)

 

 

Use and description:  

This medium is used for isolation and differentiation of Gram-negative enteric bacteria based on lactose fermentation. Eosin inhibits enterococci. E. coli produce high amount of acid due to lactose fermentation which cause amide bond between eosin Y and methylene blue that gives metallic shine to it’s colonies. Bacteria that do not ferment lactose appear as colorless or transparent, light purple colonies.

 

Composition per liter:

Peptones…………………………………….10.000g

Agar…………………………………………15.000g

Lactose……………………………………...10.000g

Dipotassium hydrogen phosphate……………2.000g

Eosin Y……………………………………....0.400g

Methylene blue………………………………0.065g

 

Final pH of the ready to use medium:   6,8 +/- 0.2

 

Medium preparation:  

Add 37.5 grams of dehydrated culture medium to 1 liter of distilled water. Autoclave for 15 min at 121oC. Cool to 45-50oC, homogenize perfectly and pour into Petri dishes. Needs 24 hours aging before use. Store dishes in the dark.

 

Quality specifications:    

Dehydrated medium:  homogeneous, light violet fine powder.                     ­­

Ready to use medium: Clear , dark brownish-violet medium

 

Microbiological response:

 

Organism

Result

Escherichia coli ATCC 25922

Growth

Enterobacter aerogenes ATCC 13048

Growth

Proteus vulgaris ATCC 13315

Growth

Staphylococcus aureus ATCC 25923

Inhibited

 

Storage:  

Dehydrated medium should be stored between 10 to 25o

فروشنده: آزمیران
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