تریپتیک سوی آگارTSA ساخت شرکت میکرومدیا مجارستان 500 گرمی


Description of MM0107

    

         TSA

         (Tryptone Soy Agar)

 

 

Use and description:  

Nutrient medium for a wide range of microorganisms. The formula conforms to the United States Pharmacopeia for sterility testing. A blood agar medium can be prepared with the addition of 5 to 10% sterile defibrinated sheep blood and can be used for the detemination of haemolytic reactions of pathogenic microorganisms in clinical specimens.

 

Composition per liter:

Sodium chloride…………………………….5.0 g

Agar………………………………………..14.0 g

Soy peptone…………………………………5.0 g

Tryptone…………………………………...15.0 g

 

Final pH of the ready to use medium:   7.3 ± 0.2

 

Medium preparation:  

Add 39 grams of dehydrated culture medium to 1 liter of distilled water. Heat with repeated stirring and boil for one minute to dissolve completely. Distribute and autoclave for 15 min at 121oC.

 

Quality specification:

Dehydrated medium: homogeneous, beige, fine powder.

Ready to use medium: amber coloured, slightly opalescent agar.

 

Microbiological response:

 

Organism

Result

Escherichia coli ATCC 25922

Good to excellent growth

Staphylococcus aureus ATCC 25923

Good to excellent growth

Pseudomonas aeruginosa ATCC 10145

Good to excellent growth

Salmonella typhimurium ATCC 14028

Good to excellent growth

 

Storage:  

Dehydrated medium should be stored between 10 to 25oC. Once opened, place the container in a dry, dark place. The dehydrated medium should not be used if there is any lump or the color has changed from the original.





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