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هکتون انتریک آگار 500 گرمی میکرومدیا مجارستان
Description of MM0207
Hektoen Enteric Agar
Use and description: For the isolation and cultivation of Gram-negative enteric microorganisms (Salmonella and Shigella) from clinical specimens. Salicin, sucrose and lactose fermenting bacteria appear as yellow to orange colonies. H2S producing organisms appear as colonies with black centers because of the presence of ferric citrate which forms iron sulphide. Bile salts inhibits most of Gram positive bacteria. Non fermenting Salmonella and Shigella colonies are blue green. The additional carbohydrate (sucrose) and salicin with the high concentration of peptones give better growth of Salmonella and Shigella spp.
Composition per liter:
Peptones…………………………………………11.500g
Yeast extract……………………………………...3.000g
Sodium chloride……………………………….....5.000g
Agar……………………………………………..12.000g
Lactose………………………………………….10.000g
Saccharose……………………………………....10.000g
Bile salt no. 3.……………………………………9.000g
Sodium thiosulphate……………………………..5.000g
Salicin……………………………………………2.000g
Ferric ammonium citrate………………………...1.500g
Acid fuchsin……………………………………..0.100g
Bromthymol blue………………………………..0.064g
Final pH of the ready to use medium: 7.5 ± 0.2
Medium preparation: Add 69.1 grams of dehydrated culture medium to 1 liter of distilled water. Heat with repeated stirring and boil for one minute to dissolve completely. DO NOT AUTOCLAVE OR OVERHEAT THIS MEDIUM. Pour plates can be prepared after the medium has cooled to 45-50oC.
Quality specifications: Dehydrated medium: homogeneous, light greenish-beige fine powder. Ready to use medium: clear to trace hazy and light to dark green in color.
Microbiological response:
Organism | Result |
Enterobacter aerogenes ATCC 13048 | Growth/yellow to salmon colored colonies with bile precipitate |
Escherichia coli ATCC 25922 | Inhibited/yellow-orange bile |
Proteus vulgaris ATCC 13315 | Inhibited/clear to yellowish |
Salmonella typhimurium ATCC 14028 | Growth/green black centers |
Shigella flexneri ATCC 12022 | Growth/blue-green colonies |
Storage: Dehydrated medium should be stored between 10 to 25oC. Once opened, place the container in a dark, dry place. The dehydrated medium should not be used if there is any lump or the color has changed from the original.
فروشنده: فروشگاه
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