آزمیران
دستگاهای آزمایشگاهی
مواد شیمیایی
شیشه آلات
محیط کشتهای میکروبی
فیلتر یا کاغذ صافی
ملزومات
سکوبندی
مقالات و توضیحات
جستجوهای مرتبط
تایوگلیکولات فلوید مدیا ساخت شرکت میکرومدیا مجارستان 500 گرمی
Description of MM0069
|
Thioglycollate Medium USP
Use and description: For the cultivation of anaerobic, microaerophilic and aerobic microorganisms in sterility testing formulation recommended by USP. The medium can be used for confirmation test sin the enumeration of Clostridium perfringens in food products. The reducing agents (thioglycollate, cystine) decrease the redox potentional and cystine helps the growth of fastidious anaerobes. It also neutrolizes arsenic, mercury and other heavy metal compounds. Resazurine is used as oxidation-reduction indicator; the medium is colourless int he reduced state and becomes pink when oxidised.
Composition per liter: Peptones…………………………………….15.000 g Glucose………………………………………5.500 g Yeast extract…………………………………5.000 g Sodium chloride……………………………...2.500 g Agar………………………………………….0.750 g L-cystine……………………………………..0.500 g Sodium thioglycollate………………………..0.500 g Resazurine……………………………………0.001 g
Final pH of the ready to use medium: 7.1 ± 0.2
Medium preparation: Add 30.0 grams of dehydrated culture medium to 1 liter of distilled water. Mix thoroughly. Gently heat and bring to the boiling. Distribute into tubes or flasks. Autoclave for 15 min at 121oC. If the upper portion of the is pink becouse of oxidaton, anaerobic mconditions can be restored (once only) by heating in a boiling water bath or by steam.
Quality specifications: Dehydrated medium: homogeneous, cream-white fine powder. Ready to use medium: straw-coloured, clear fluid.
Microbiological response:
Storage: Dehydrated medium should be stored between 10 to 25oC. Once opened, place the container in a dark, dry place. The dehydrated medium should not be used if there is any lump or the color has changed from the original.
|
فروشنده: فروشگاه
گارانتی اصالت و سلامت کالا
پشتیبانی ۷ روز هفته ۲۴ ساعته
۰ تومان
(موجود نیست)
محصولات مرتبط
:
