تی اس آی TSI ساخت شرکت میکرومدیا مجارستان 500 گرمی

 

جهت دریافت کاتالوگ لطفا روی عکس کلیک کنید

Description of MM0204

 

                                               TSI Agar

              (Triple Sugar Iron Agar)

 

Use and description:  

Medium used for the biochemical characterisation of enterobacteria in general and of Salmonella in particular int he analysis of food products and in screening for contamination of non-sterile pharmaceutical products. The principle of the medium relies ont he ability of enterobacteria to ferment glucose (with or wothout production of gas), lactose, and saccharose and to reduce sulphates to sulphides which produces a black precipitate of iron sulphide.

 

Composition per liter:

Sodium chloride………………………………5.000 g

Agar………………………………………….12.000 g

Peptone………………………………………20.000 g

Meat extract…………………………………...3.000 g

Yeast extract…………………………………..3.000 g

Lactose……………………………………… 10.000 g

Saccharose...………………………………….10.000 g

Glucose………………………………………..1.000 g

Ferric citrate…………………………………...0.300 g

Sodium thiosulphate…………………………..0.300 g

Phenol red……………………………………..0.025 g                                                   

Final pH of the ready to use medium:   7.4 ± 0.2

 

Medium preparation:  

Add 65 grams of dehydrated culture medium to 1 liter of distilled water. Mix well and bring to the boil to dissolve completely. Distribute and sterilize by autoclaving for 15 min at 121oC. Allow the medium to set in sloped form with a butt about 2,5 cm deep.

 

Quality specification:

Dehydrated medium: homogeneous, light beige to pinkish beige, fine powder.

Ready to use medium: red-orange and clear to trace hazy.

 

Microbiological response:

 

Organism

Result (Slant/Butt/Gas/H2S)

Escherichia coli ATCC 25922

A/A/+/-

Pseudomonas aeruginosa ATCC 27853

K/K/-/-

Salmonella typhimurium ATCC 14028

K/A/-/+

Proteus mirabilis ATCC 12453

K/A/-/+

Shigella flexneri ATCC 12022

K/A/-/-

 

 

Typical reactions:

 

Organism

Butt

Slope

H2S

Enterobacter aerogenes

AG

    A    

-

Enterobacter cloacae

   AG  

A

-

Escherichia coli

AG

A

-

Proteus vulgaris

AG

A

+

Morganella morgianii

A or AG

NC or ALK

-

Shigella dysenteriae

A

NC or ALK

-

Shigella sonnei

A

NC or ALK

-

Salmonella typhi

A

NC or ALK

+

Salmonella paratyphi

AG

NC or ALK

-

Salmonella enteritidis

AG

NC or ALK

+



فروشنده: آزمیران
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